APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1μg/ml |
| 2.5μg/ml |
|
Note :
IHC-P
Human Thymus shows nuclear staining in select thymocytes. This product was confirmed in IHC on fetal Pig Thymus (PMID: 24770647).
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89336-PEP).
Product Note
This antibody is expected to recognise isoform 1 (NP_000374.1) only.
Predict Reactivity
Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KDVDLSQPRKGRKP, from the internal region of the protein sequence according to NP_000374.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
autoimmune regulator , AIRE1 , APECED , APS1 , APSI , PGA1
Cellular Localization
Nucleus , Cytoplasm
Background
This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED). [provided by RefSeq, Jun 2012]
Database
DATA IMAGES
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GTX89336 IHC-P Image
IHC-P analysis of human thymus using GTX89336 AIRE (isoform 1) antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX89336 WB Image
WB analysis of HEK293 cell lysates untransfected (Lane 3) and transfected with human AIRE (lane 4) using GTX89336 AIRE (isoform 1) antibody, Internal.
Dilution : 0.05μg/ml
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REFERENCE
There are currently no references for AIRE (isoform 1) antibody, Internal (GTX89336). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for AIRE (isoform 1) antibody, Internal (GTX89336). Be the first to share your experience with this product.
