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AKT1 antibody [rAKT1/2491]

Anti-AKT1 antibody [rAKT1/2491] used in IHC (Paraffin sections) (IHC-P). GTX02583
Anti-AKT1 antibody [rAKT1/2491] used in Western Blot (WB). GTX02583
Anti-AKT1 antibody [rAKT1/2491] used in IHC (Paraffin sections) (IHC-P). GTX02583

Cat. No. GTX02583

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 rAKT1/2491

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($529)
See all AKT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
IHC-P 1-2 μg/ml

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human AKT1 protein (around aa 85-189)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-AKT1 antibody [rAKT1/2491] used in IHC (Paraffin sections) (IHC-P). GTX02583

GTX02583 IHC-P Image

IHC-P analysis of human prostate carcinoma section using GTX02583 AKT1 antibody [rAKT1/2491].

Anti-AKT1 antibody [rAKT1/2491] used in Western Blot (WB). GTX02583

GTX02583 WB Image

WB analysis of MCF-7 whole cell lysate using GTX02583 AKT1 antibody [rAKT1/2491].

Anti-AKT1 antibody [rAKT1/2491] used in IHC (Paraffin sections) (IHC-P). GTX02583

GTX02583 IHC-P Image

IHC-P analysis of human colon carcinoma section using GTX02583 AKT1 antibody [rAKT1/2491].

REFERENCE

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REVIEW

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Package List Price ($)
$ 529