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ANKLE2 antibody [N1N3]

Anti-ANKLE2 antibody [N1N3] used in Western Blot (WB). GTX120698
Anti-ANKLE2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX120698

Cat. No. GTX120698

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ANKLE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ANKLE2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722701

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ankyrin repeat and LEM domain containing 2 , KIAA0692 , LEMD7 , Lem4 , MCPH16

Cellular Localization

Membrane

Database

Research Area

DATA IMAGES

Anti-ANKLE2 antibody [N1N3] used in Western Blot (WB). GTX120698

GTX120698 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX120698 diluted at 1:1000

Anti-ANKLE2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX120698

GTX120698 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ANKLE2(GTX120698) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Rose M et al. Front Oncol 2024; 14 : 1222698 The expression and role of the Lem-D proteins Ankle2, Emerin, Lemd2, and TMPO in triple-negative breast cancer cell growth.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169