APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Neuro2A , GL261 , C8D30 , NIH-3T3 , C2C12 , PC-12 , Rat-2 , 293T , A431 , HeLa , HepG2
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ARHGEF18. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888157
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rho/Rac guanine nucleotide exchange factor 18 , P114-RhoGEF , RP78 , SA-RhoGEF , p114RhoGEF
Cellular Localization
Cytoplasm
Background
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
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GTX629806 WB Image
ARHGEF18 antibody [GT1972] detects ARHGEF18 protein by Western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat-2 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [GT1972] (GTX629806) dilution: 1:1000
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GTX629806 IHC-P Image
ARHGEF18 antibody [GT1972] detects ARHGEF18 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat lymph node.ARHGEF18 stained by ARHGEF18 antibody [GT1972] (GTX629806) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629806 WB Image
ARHGEF18 antibody [GT1972] detects ARHGEF18 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [GT1972] (GTX629806) dilution: 1:3000
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GTX629806 IHC-P Image
ARHGEF18 antibody [GT1972] detects ARHGEF18 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.ARHGEF18 stained by ARHGEF18 antibody [GT1972] (GTX629806) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629806 WB Image
ARHGEF18 antibody [GT1972] detects ARHGEF18 protein by Western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extract E. 30 μg C2C12 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [GT1972] (GTX629806) dilution: 1:1000
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REFERENCE
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