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ATF6 antibody

Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30071
Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30071
Anti-ATF6 antibody used in Western Blot (WB). GTX30071
Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30071
Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX30071
Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX30071
Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX30071

Cat. No. GTX30071

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ATF6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Product Note

The immunogen sequence of this antibody is within the cleaved form of ATF6 alpha.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ATF6 (NP_031374.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 6 , ACHM7 , ATF6A

Background

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30071

GTX30071 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX30071 ATF6 antibody.
Dilution : 1:100

Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30071

GTX30071 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX30071 ATF6 antibody.
Dilution : 1:100

Anti-ATF6 antibody used in Western Blot (WB). GTX30071

GTX30071 WB Image

WB analysis of various cell lysates using GTX30071 ATF6 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30071

GTX30071 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX30071 ATF6 antibody.
Dilution : 1:100

Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX30071

GTX30071 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX30071 ATF6 antibody.
Dilution : 1:50

Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX30071

GTX30071 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX30071 ATF6 antibody.
Dilution : 1:50

Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX30071

GTX30071 IHC-P Image

IHC-P analysis of human colon tissue using GTX30071 ATF6 antibody.
Dilution : 1:50

REFERENCE

There are currently no references for ATF6 antibody (GTX30071). Be the first to share your publications with this product.
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REVIEW

ATF6 antibody Cat. No. GTX30071

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-ATF6 antibody used in Western Blot (WB). GTX30071
Date : Anonymous submitted on 14-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : EA.hy926 cell
Amount used : 10ug
Blocking : 5% skim-milk in TBS, 4°C, 16Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399