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ATG10 antibody

Anti-ATG10 antibody used in IHC (Paraffin sections) (IHC-P). GTX55523
Anti-ATG10 antibody used in IHC (Paraffin sections) (IHC-P). GTX55523
Anti-ATG10 antibody used in IHC (Paraffin sections) (IHC-P). GTX55523
Anti-ATG10 antibody used in Western Blot (WB). GTX55523

Cat. No. GTX55523

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ATG10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ATG10 (NP_113670.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 10 , APG10 , APG10L , pp12616

Cellular Localization

Cytoplasm

Background

Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-ATG10 antibody used in IHC (Paraffin sections) (IHC-P). GTX55523

GTX55523 IHC-P Image

IHC-P analysis of rat testis tissue using GTX55523 ATG10 antibody.
Dilution : 1:100

Anti-ATG10 antibody used in IHC (Paraffin sections) (IHC-P). GTX55523

GTX55523 IHC-P Image

IHC-P analysis of human colon tissue using GTX55523 ATG10 antibody.
Dilution : 1:100

Anti-ATG10 antibody used in IHC (Paraffin sections) (IHC-P). GTX55523

GTX55523 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX55523 ATG10 antibody.
Dilution : 1:100

Anti-ATG10 antibody used in Western Blot (WB). GTX55523

GTX55523 WB Image

WB analysis of mouse small intestine tissue lysate using GTX55523 ATG10 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399