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ATGL antibody, Internal

Anti-ATGL antibody, Internal used in Western Blot (WB). GTX89133
Anti-ATGL antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89133

Cat. No. GTX89133

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all ATGL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-2μg/ml
IHC-P 2-3μg/ml

Note :

IHC-P
Human Heart shows intense staining of distinct spots in the myocardial fibres in transverse section.
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89133-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NIIEVSKEARKR, from the internal region of the protein sequence according to NP_065109.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

patatin like phospholipase domain containing 2 , 1110001C14Rik , ATGL , FP17548 , PEDF-R , TTS-2.2 , TTS2 , iPLA2zeta

Cellular Localization

Cell membrane; Single-pass type II membrane protein

Background

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-ATGL antibody, Internal used in Western Blot (WB). GTX89133

GTX89133 WB Image

WB analysis of human adipose lysate using GTX89133 ATGL antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-ATGL antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89133

GTX89133 IHC-P Image

IHC-P analysis of human heart using GTX89133 ATGL antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399