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Annexin XI antibody [1C6]

KO/KD Overexpression
Anti-Annexin XI antibody [1C6] used in Western Blot (WB). GTX84884
Anti-Annexin XI antibody [1C6] used in Flow cytometry (FACS). GTX84884
Anti-Annexin XI antibody [1C6] used in Western Blot (WB). GTX84884
Anti-Annexin XI antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84884
Anti-Annexin XI antibody [1C6] used in Western Blot (WB). GTX84884

Cat. No. GTX84884

2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1C6

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, IP

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all Annexin XI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ANXA11 (NP_001148) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A11 , ALS23 , ANX11 , CAP-50 , CAP50

Cellular Localization

Cytoplasm,nucleoplasm

Background

Binds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis.

Database

Research Area

DATA IMAGES

Anti-Annexin XI antibody [1C6] used in Western Blot (WB). GTX84884

GTX84884 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Annexin XI plasmid (Right) or empty vector (Left) for 48 hrs using GTX84884 Annexin XI antibody [1C6].
Loading : 5 ug per lane

Anti-Annexin XI antibody [1C6] used in Flow cytometry (FACS). GTX84884

GTX84884 FACS Image

FACS analysis of HEK293T cells transfected with either Annexin XI plasmid(Red) or empty vector control plasmid(Blue) using GTX84884 Annexin XI antibody [1C6].

Anti-Annexin XI antibody [1C6] used in Western Blot (WB). GTX84884

GTX84884 WB Image

WB analysis of various cell lines using GTX84884 Annexin XI antibody [1C6].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Annexin XI antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84884

GTX84884 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Annexin XI plasmid using GTX84884 Annexin XI antibody [1C6].

Anti-Annexin XI antibody [1C6] used in Western Blot (WB). GTX84884

GTX84884 WB Image

KO/KD

WB analysis of wild type and Annexin XI knock out 293T cell lysate(10μg per lane) using GTX84884 Annexin XI antibody [1C6].
Dilution : 1:500

REFERENCE

There are currently no references for Annexin XI antibody [1C6] (GTX84884). Be the first to share your publications with this product.
Submit a Reference

REVIEW

Annexin XI antibody [1C6] Cat. No. GTX84884

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Annexin XI antibody [1C6] used in Immunoprecipitation (IP). GTX84884
Date : Anonymous submitted on 07-Jun-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HeLa cells
Lane Description :
SM: 10% starting material
UB:10% unbound fraction
IP: IP antibody
Figure Note : Antibody-bead conjugates were prepared by adding 1.0 μg of antibody to 500 ul of PBS with 0.01% triton X-100, together with 30μl of protein A-Sepharose beads. One ml aliquots at 1.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Annexin XI antibody [1C6] used in Western Blot (WB). GTX84884
Date : Anonymous submitted on 01-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa WT and ANXA11 KO cells
Amount used : 50μg
Primary Antibody : 1:200, °C, Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519