PRODUCT
Summary
Anterior gradient 2 (AGR2), also known as hAG-2 and Gob-4, is a member of the disulfide isomerase family of endoplasmic reticulum proteins with a predicted molecular weight of 20 kDa. AGR2 is overexpressed in human mucinous ovarian tumor tissues and in patient sera with early stage mucinous ovarian tumors. It was found to enhance tumor growth and metastasis, and is a potential tumor marker for detection of circulating tumor cells.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Anterior Gradient 2. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888413
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
anterior gradient 2, protein disulphide isomerase family member , AG-2 , AG2 , GOB-4 , HAG-2 , HEL-S-116 , HPC8 , PDIA17 , XAG-2
Cellular Localization
Secreted
Database
Research Area
DATA IMAGES
![Anti-Anterior Gradient 2 antibody [GT5812] used in Western Blot (WB). GTX634163](/templates/default/js/lightbox/images/loading.gif "Anti-Anterior Gradient 2 antibody [GT5812] used in Western Blot (WB). GTX634163")
|
GTX634163 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
|
GTX634163 IHC-P Image
Anterior Gradient 2 antibody [GT5812] detects secreted Anterior Gradient 2 protein by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX634163 ICC/IF Image
Anterior Gradient 2 antibody [GT5812] detects Anterior Gradient 2 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:500.Blue: Hoechst 33342 staining.
|
|
|
GTX634163 IHC-P Image
Anterior Gradient 2 antibody [GT5812] detects secreted Anterior Gradient 2 protein by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
REFERENCE
REVIEW
There are currently no reviews for Anterior Gradient 2 antibody [GT5812] (GTX634163). Be the first to share your experience with this product.
![Anti-Anterior Gradient 2 antibody [GT5812] used in Western Blot (WB). GTX634163](/upload/website/prouct_img/small/GTX634163/GTX634163_42849_20170511_WB_w_23061202_819.webp "Anti-Anterior Gradient 2 antibody [GT5812] used in Western Blot (WB). GTX634163")
![Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163](/upload/website/prouct_img/small/GTX634163/GTX634163_42849_20180504_IHC-P_1_w_23061202_464.webp "Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163")
![Anti-Anterior Gradient 2 antibody [GT5812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634163](/upload/website/prouct_img/small/GTX634163/GTX634163_42849_20180523_ICC_IF_w_23061202_994.webp "Anti-Anterior Gradient 2 antibody [GT5812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634163")
![Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163](/upload/website/prouct_img/small/GTX634163/GTX634163_42849_20180504_IHC-P_w_23061202_607.webp "Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163")
![Anti-Anterior Gradient 2 antibody [GT5812] used in Western Blot (WB). GTX634163](/upload/website/prouct_img/normal/GTX634163/GTX634163_42849_20170511_WB_w_23061202_819.webp "Anti-Anterior Gradient 2 antibody [GT5812] used in Western Blot (WB). GTX634163")
![Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163](/upload/website/prouct_img/normal/GTX634163/GTX634163_42849_20180504_IHC-P_1_w_23061202_464.webp "Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163")
![Anti-Anterior Gradient 2 antibody [GT5812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634163](/upload/website/prouct_img/normal/GTX634163/GTX634163_42849_20180523_ICC_IF_w_23061202_994.webp "Anti-Anterior Gradient 2 antibody [GT5812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634163")
![Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163](/upload/website/prouct_img/normal/GTX634163/GTX634163_42849_20180504_IHC-P_w_23061202_607.webp "Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163")