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Asporin antibody [N3C1], Internal

Anti-Asporin antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104790
Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790

Cat. No. GTX104790

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

mouse liver , rat heart

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Asporin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240484

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

asporin , OS3 , PLAP-1 , PLAP1 , SLRR1C

Background

ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin (MIM 125255) (Lorenzo et al., 2001 [PubMed 11152692]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Asporin antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104790

GTX104790 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Asporin(GTX104790) antibody at 1:200 dilution.

Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790

GTX104790 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal (GTX104790) diluted at 1:500.

Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790

GTX104790 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal (GTX104790) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790

GTX104790 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal (GTX104790) diluted at 1:500.

Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790

GTX104790 WB Image

The data was published in the 2022 in Clin Exp Dent Res. PMID: 35106969

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169