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BARD1 antibody

Orthogonal
Anti-BARD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132094
Anti-BARD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132094
Anti-BARD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132094
Anti-BARD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132094
Anti-BARD1 antibody used in Western Blot (WB). GTX132094

Cat. No. GTX132094

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BARD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BARD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886564

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRCA1 associated RING domain 1

Cellular Localization

Nucleus

Background

This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BARD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132094

GTX132094 IHC-P Image

BARD1 antibody detects BARD1 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
BARD1 antibody (GTX132094) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BARD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132094

GTX132094 ICC/IF Image

BARD1 antibody detects BARD1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BARD1 protein stained by BARD1 antibody (GTX132094) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-BARD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132094

GTX132094 IHC-P Image

BARD1 antibody detects BARD1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
BARD1 stained by BARD1 antibody (GTX132094) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BARD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132094

GTX132094 IHC-P Image

BARD1 antibody detects BARD1 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
BARD1 antibody (GTX132094) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BARD1 antibody used in Western Blot (WB). GTX132094

GTX132094 WB Image

Orthogonal

A431 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BARD1 antibody (GTX132094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zhu Q et al. Nat Commun 2021; 12 (1) : 6653 RNF19A-mediated ubiquitination of BARD1 prevents BRCA1/BARD1-dependent homologous recombination.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169