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BTK antibody

Anti-BTK antibody used in IHC (Paraffin sections) (IHC-P). GTX31694
Anti-BTK antibody used in Western Blot (WB). GTX31694
Anti-BTK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31694
Anti-BTK antibody used in IHC (Paraffin sections) (IHC-P). GTX31694
Anti-BTK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31694

Cat. No. GTX31694

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all BTK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
ICC/IF 10 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

Daudi Cell Lysate(GTX23951) , U937 Cell Lysate(GTX23959)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK.The immunogen is located within amino acids 20 - 70 of BTK.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Bruton tyrosine kinase , AGMX1 , AT , ATK , BPK , IGHD3 , IMD1 , PSCTK1 , XLA

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES

Anti-BTK antibody used in IHC (Paraffin sections) (IHC-P). GTX31694

GTX31694 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31694 BTK antibody.
Working concentration : 20 μg/ml

Anti-BTK antibody used in Western Blot (WB). GTX31694

GTX31694 WB Image

WB analysis of U937 cell lysate using GTX31694 BTK antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-BTK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31694

GTX31694 ICC/IF Image

ICC/IF analysis of Daudi cells using GTX31694 BTK antibody.
Working concentration : 10 μg/ml

Anti-BTK antibody used in IHC (Paraffin sections) (IHC-P). GTX31694

GTX31694 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31694 BTK antibody.
Working concentration : 5 μg/ml

Anti-BTK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31694

GTX31694 ICC/IF Image

ICC/IF analysis of Daudi cells using GTX31694 BTK antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399