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Bad antibody [GT1175]

Recombinant antibody
Anti-Bad antibody [GT1175] used in Western Blot (WB). GTX09508
Anti-Bad antibody [GT1175] used in IHC (Paraffin sections) (IHC-P). GTX09508
Anti-Bad antibody [GT1175] used in Western Blot (WB). GTX09508

Cat. No. GTX09508

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1175

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Bad products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan Bad

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated agonist of cell death , BBC2 , BCL2L8

Cellular Localization

Mitochondrion outer membrane,Cytoplasm

Background

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bad antibody [GT1175] used in Western Blot (WB). GTX09508

GTX09508 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bad antibody [GT1175] (GTX09508) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bad antibody [GT1175] used in IHC (Paraffin sections) (IHC-P). GTX09508

GTX09508 IHC-P Image

IHC-P analysis of hmuan placenta tissue section using GTX09508 Bad antibody [GT1175].
Dlution : 1:100

Anti-Bad antibody [GT1175] used in Western Blot (WB). GTX09508

GTX09508 WB Image

WB analysis of various samples using GTX09508 Bad antibody [GT1175].
Dilution : 1:1000
Loading : 25 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399