Bcl-2 antibody [8E12]

Cat. No. GTX82803

Host	Mouse
Clonality	Monoclonal
Clone Name	8E12
Isotype	IgG1
Application	ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX82803 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 apoptosis regulator , Bcl-2 , PPP1R50

Cellular Localization

Mitochondrion outer membrane,Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

Database

Gene ID: 596 BCL2
UniProt: P10415 BCL2

Research Area

DATA IMAGES

GTX82803 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX82803 Bcl-2 antibody [8E12].
Green : Bcl-2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX82803 FACS Image

FACS analysis of 3T3L1 cells using GTX82803 Bcl-2 antibody [8E12].
Green : Bcl-2
Purple : negative control

GTX82803 IHC-P Image

IHC-P analysis of colon cancer tissue (left) and human brain tissue (right) using GTX82803 Bcl-2 antibody [8E12].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

Bcl-2 antibody [8E12]

Cat. No. GTX82803

Host

Clonality

Clone Name

Isotype

Application

Reactivity