APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:250-1:500 |
1:250-1:500 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT1B5 is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Bcl-4 protein.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL2 apoptosis regulator , BCL2 , BCL2, apoptosis regulator , Bcl2 , PPP1R50 , Bcl-2
Cellular Localization
Mitochondrion outer membrane,Nucleus membrane,Endoplasmic reticulum membrane
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
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GTX57574 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57574 Bcl-2 antibody. Blue: Hoechst 3342 Red: Primary antibody Dilution: 1:500
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GTX57574 WB Image
WB analysis of NIH3T3 whole cell lysate using GTX57574 Bcl-2 antibody. Loading : 35 μg Dilution : 1:500
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GTX57574 FACS Image
FACS analysis of HeLa cells using GTX57574 Bcl-2 antibody. Cell Number: 1 x 10⁶ cells Primary antibody: Red line Antibody amount: 2-5 μg
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REFERENCE
There are currently no references for Bcl-2 antibody [AT1B5] (GTX57574). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Bcl-2 antibody [AT1B5] (GTX57574). Be the first to share your experience with this product.