APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
1-2μg/ml for 30 minutes at RT |
Assay dependent |
Note :
IHC-P
Staining of formalin-fixed tissues require heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
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Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human bcl-2 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL2 , BCL2, apoptosis regulator , Bcl2 , PPP1R50 , Bcl-2 , BCL2 apoptosis regulator
Cellular Localization
Mitochondrion outer membrane,Nucleus membrane,Endoplasmic reticulum membrane
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
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GTX17713 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/2210R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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GTX17713 IHC-P Image
IHC-P analysis of human follicular lymphoma tissue using GTX17713 Bcl-2 antibody [BCL2/2210R].
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GTX17713 IHC-P Image
IHC-P analysis of human spleen tissue using GTX17713 Bcl-2 antibody [BCL2/2210R].
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GTX17713 WB Image
WB analysis of MCF-7 cell lysate using GTX17713 Bcl-2 antibody [BCL2/2210R].
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REFERENCE
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REVIEW
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