APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human Bcl-2.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL2 apoptosis regulator , BCL2 , BCL2, apoptosis regulator , Bcl2 , PPP1R50 , Bcl-2
Cellular Localization
Mitochondrion outer membrane,Nucleus membrane,Endoplasmic reticulum membrane
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
![Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194](/templates/default/js/lightbox/images/loading.gif "Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194")
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GTX01194 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [GT1213] (GTX01194) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX01194 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [GT1213] (GTX01194) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX01194 WB Image
WB analysis of various samples using GTX01194 Bcl-2 antibody [GT1213].
Dilution : 1:1000
Loading : 25μg
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GTX01194 IP Image
IP analysis of Mouse lung tissue lysate using GTX01194 Bcl-2 antibody [GT1213].
Total protein amount : 200μg
IP antibody : 3μg
Dilution : 1:1000
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GTX01194 IHC-P Image
IHC-P analysis of human placenta tissue section using GTX01194 Bcl-2 antibody [GT1213].
Dilution : 1:100
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REFERENCE
There are currently no references for Bcl-2 antibody [GT1213] (GTX01194). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Bcl-2 antibody [GT1213] (GTX01194). Be the first to share your experience with this product.
![Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194](/upload/website/prouct_img/small/GTX01194/GTX01194_40000000173_20200313_WB_TPM_watermark_w_23053121_244.webp "Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194")
![Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194](/upload/website/prouct_img/small/GTX01194/GTX01194_40000000173_20200313_WB_w_23053121_430.webp "Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194")
![Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194](/upload/website/prouct_img/small/GTX01194/GTX01194_20200327_WB_1_w_23053121_322.webp "Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194")
![Anti-Bcl-2 antibody [GT1213] used in Immunoprecipitation (IP). GTX01194](/upload/website/prouct_img/small/GTX01194/GTX01194_20200327_IP_1_w_23053121_824.webp "Anti-Bcl-2 antibody [GT1213] used in Immunoprecipitation (IP). GTX01194")
![Anti-Bcl-2 antibody [GT1213] used in IHC (Paraffin sections) (IHC-P). GTX01194](/upload/website/prouct_img/small/GTX01194/GTX01194_20200327_IHC-P_23_w_23053121_679.webp "Anti-Bcl-2 antibody [GT1213] used in IHC (Paraffin sections) (IHC-P). GTX01194")
![Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194](/upload/website/prouct_img/normal/GTX01194/GTX01194_40000000173_20200313_WB_TPM_watermark_w_23053121_244.webp "Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194")
![Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194](/upload/website/prouct_img/normal/GTX01194/GTX01194_40000000173_20200313_WB_w_23053121_430.webp "Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194")
![Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194](/upload/website/prouct_img/normal/GTX01194/GTX01194_20200327_WB_1_w_23053121_322.webp "Anti-Bcl-2 antibody [GT1213] used in Western Blot (WB). GTX01194")
![Anti-Bcl-2 antibody [GT1213] used in Immunoprecipitation (IP). GTX01194](/upload/website/prouct_img/normal/GTX01194/GTX01194_20200327_IP_1_w_23053121_824.webp "Anti-Bcl-2 antibody [GT1213] used in Immunoprecipitation (IP). GTX01194")
![Anti-Bcl-2 antibody [GT1213] used in IHC (Paraffin sections) (IHC-P). GTX01194](/upload/website/prouct_img/normal/GTX01194/GTX01194_20200327_IHC-P_23_w_23053121_679.webp "Anti-Bcl-2 antibody [GT1213] used in IHC (Paraffin sections) (IHC-P). GTX01194")