Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5-4 μg/ml |
20 μg/ml |
Not tested in other applications.
Calculated MW
Product Note
The antibody detects the 30 kDa Bcl-xL protein. It does not react with other Bcl-2 family members.
Form
Liquid
Buffer
PBS, 0.5% BSA, 30% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide surrounding amino acid 56 of human Bcl-xL
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
RRID
AB_10722492
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
BCL2 like 1 , BCL2L , BCL2L1 , BCLX , BCLXL/S , BclX , PPP1R52 , Bcl-X
Cellular Localization
Mitochondrion inner membrane,Mitochondrion outer membrane,Mitochondrion matrix,Cytoplasmic vesicle,Secretory vesicle,Synaptic vesicle membrane,Cytoplasm,Cytosol,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Nucleus membrane
Background
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]
Database
Research Area