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Bid antibody [23F7]

Anti-Bid antibody [23F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15667
Anti-Bid antibody [23F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15667
Anti-Bid antibody [23F7] used in Western Blot (WB). GTX15667
Anti-Bid antibody [23F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15667
Anti-Bid antibody [23F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15667

Cat. No. GTX15667

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 23F7

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Bid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1-2 μg/ml
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Residues 5-195 from human recombinant protein expressed in bacteria

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BH3 interacting domain death agonist , FP497

Cellular Localization

Cytoplasm,Mitochondrion outer membrane

Background

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bid antibody [23F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15667

GTX15667 ICC/IF Image

ICC/IF analysis of A549 cells using GTX15667 Bid antibody [23F7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4ºC

Anti-Bid antibody [23F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15667

GTX15667 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX15667 Bid antibody [23F7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4ºC

Anti-Bid antibody [23F7] used in Western Blot (WB). GTX15667

GTX15667 WB Image

WB analysis of 0.125-1ug purified BID-GST fusion protein using GTX15667 Bid antibody [23F7].
Dilution : 1:1000

Anti-Bid antibody [23F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15667

GTX15667 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15667 Bid antibody [23F7]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-Bid antibody [23F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15667

GTX15667 ICC/IF Image

ICC/IF analysis of Hela cells using GTX15667 Bid antibody [23F7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399