APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-3μg/ml |
2-5μg/ml |
Note :
IHC-P
Human Spleen shows nuclear staining.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89813-PEP).
Predict Reactivity
Mouse, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QRKTIWVDEDQL, from the C Terminus of the protein sequence according to NP_115791.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
caspase recruitment domain family member 11 , BENTA , BIMP3 , CARMA1 , IMD11 , IMD11A , PPBL
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89813 IHC-P Image
IHC-P analysis of human spleen using GTX89813 CARD11 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 2μg/ml
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GTX89813 WB Image
WB analysis of Jurkat lysate using GTX89813 CARD11 antibody, C-term. Dilution : 1μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for CARD11 antibody, C-term (GTX89813). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CARD11 antibody, C-term (GTX89813). Be the first to share your experience with this product.