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CD11a antibody, Internal

Anti-CD11a antibody, Internal used in Western Blot (WB). GTX88390
Anti-CD11a antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88390
Anti-CD11a antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88390

Cat. No. GTX88390

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all CD11a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-2μg/ml
IHC-P 3.75μg/ml

Note :

IHC-P
Human Lymph Node shows membrane staining in the paracortex.
Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88390-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-YQVRIQPSIHDHN, from the internal region of the protein sequence according to NP_002200.2; NP_001107852.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha L , CD11A , LFA-1 , LFA1A

Cellular Localization

Cell membrane

Background

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD11a antibody, Internal used in Western Blot (WB). GTX88390

GTX88390 WB Image

WB analysis of human spleen lysate using GTX88390 CD11a antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-CD11a antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88390

GTX88390 IHC-P Image

IHC-P analysis of human lymph node using GTX88390 CD11a antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

Anti-CD11a antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88390

GTX88390 IHC-P Image

IHC-P analysis of human spleen using GTX88390 CD11a antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399