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CD11b antibody [GT0001]

Anti-CD11b antibody [GT0001] used in Flow cytometry (FACS). GTX628077

Cat. No. GTX628077

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT0001

Isotype

 IgG2b

Application

 FACS

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD11b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human CD11b.

Purification

Affinity purified by Protein G.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888017

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha M , CD11B , CR3A , MAC-1 , MAC1A , MO1A , SLEB6

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD11b antibody [GT0001] used in Flow cytometry (FACS). GTX628077

GTX628077 FACS Image

CD11b antibody [GT0001] (GTX628077) detects CD11b/ITGAM protein by flow cytometry analysis.
Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4oC for 15 min.
Left: Unlabelled sample was used as a control.
Right: CD11b antibody [GT0001] (GTX628077) dilution: 1:50.
Acquisition of 40,000 events were collected for FACS analysis.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations