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CD18 antibody [MEM-148]

Orthogonal
Anti-CD18 antibody [MEM-148] used in Western Blot (WB). GTX28220

Cat. No. GTX28220

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-148

Isotype

 IgG1

Application

 WB, FACS, IP, Functional Assay

Reactivity

 Human
Package
100 μg ($339)
See all CD18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS Assay dependent
IP Assay dependent
Functional Assay Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Product Note

This antibody recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18.
We do not recommend use of this product for Pig samples.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peripheral blood mononuclear cells

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_371915

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD18 , ITGB2 , LAD , LCAMB , LFA1 , MAC1 , MF17 , MFI7 , integrin subunit beta 2 , Integrin beta2

Cellular Localization

Membrane

Background

This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-CD18 antibody [MEM-148] used in Western Blot (WB). GTX28220

GTX28220 WB Image

Orthogonal

WB analysis of PMA-activated neutrophils (A) and monocytes (B) using GTX28220 CD18 antibody [MEM-148].The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).

REFERENCE

Application Reference

Li N et al. J Immunol 2013; 190 (8) : 4371-81 Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation.
Application : Functional Assay
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339