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CD1a antibody [O10]

Anti-CD1a antibody [O10] used in Flow cytometry (FACS). GTX34469
Anti-CD1a antibody [O10] used in IHC (Paraffin sections) (IHC-P). GTX34469

Cat. No. GTX34469

Host

Mouse

Clonality

Monoclonal

Clone Name

O10

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Monkey
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human thymus cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD1 , CD1A , CD1a molecule , FCB6 , HTA1 , R4 , T6 , CD1a

Cellular Localization

Cell membrane,Membrane raft,Endosome membrane

Background

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-CD1a antibody [O10] used in Flow cytometry (FACS). GTX34469

GTX34469 FACS Image

FACS analysis of MOLT-4 cells using GTX34469 CD1a antibody [O10].
Blue : Primary antibody
Red : Isotype control

Anti-CD1a antibody [O10] used in IHC (Paraffin sections) (IHC-P). GTX34469

GTX34469 IHC-P Image

IHC-P analysis of human skin tissue using GTX34469 CD1a antibody [O10].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529