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CD44 antibody [F10-44-2]

Anti-CD44 antibody [F10-44-2] used in IHC (Paraffin sections) (IHC-P). GTX30725
Anti-CD44 antibody [F10-44-2] used in Flow cytometry (FACS). GTX30725
Anti-CD44 antibody [F10-44-2] used in IHC (Paraffin sections) (IHC-P). GTX30725

Cat. No. GTX30725

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 F10-44-2

Isotype

 IgG2a

Application

 ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Cynomolgus monkey
Package
200 μg ($459)
See all CD44 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1/50-1/100
IHC-Fr Assay dependent
FACS 1/10-1/20

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Product Note

This antibody recognizes an epitope on human CD44 outside the regions coded for by the variable region exons and is thus expected to recognize all isoforms of human CD44 (Mackay et al. 1994).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human T lymphocytes

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD44 antibody [F10-44-2] used in IHC (Paraffin sections) (IHC-P). GTX30725

GTX30725 IHC-P Image

IHC-P analysis of human breast cancer biopsy tissue using GTX30725 CD44 antibody [F10-44-2].

Anti-CD44 antibody [F10-44-2] used in Flow cytometry (FACS). GTX30725

GTX30725 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX30725 CD44 antibody [F10-44-2].

Anti-CD44 antibody [F10-44-2] used in IHC (Paraffin sections) (IHC-P). GTX30725

GTX30725 IHC-P Image

IHC-P analysis of human breast cancer biopsy tissue using GTX30725 CD44 antibody [F10-44-2].

REFERENCE

Clone Reference

Lindqvist U et al. Acta Derm Venereol 2012; 92 (4):372-7 Dermal distribution of hyaluronan in psoriatic arthritis; coexistence of CD44, MMP3 and MMP9.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459