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CDC25B antibody [EPR3459(2)]

Anti-CDC25B antibody [EPR3459(2)] used in Western Blot (WB). GTX63329

Cat. No. GTX63329

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 EPR3459(2)

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399)
See all CDC25B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Buffer, 0.05% BSA, 50% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues in human Cdc25B

Purification

Tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888333

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,488.

TARGET

Synonyms

cell division cycle 25B

Background

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CDC25B antibody [EPR3459(2)] used in Western Blot (WB). GTX63329

GTX63329 WB Image

WB analysis of (1) K562, (2) U937 and (3) THP-1 lysates (10 μg per lane) using Cdc25B antibody [EPR3459(2)] at a dilution of 1:1,000.

REFERENCE

Application Reference

Rodriguez A et al. Theor Biol Med Model 2015; 12 : 19 Fanconi anemia cells with unrepaired DNA damage activate components of the checkpoint recovery process.
Application : WB
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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399