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CDX2 antibody

Orthogonal
Anti-CDX2 antibody used in Western Blot (WB). GTX113160
Anti-CDX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113160
Anti-CDX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113160

Cat. No. GTX113160

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CDX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HCT116 , HCT116 nuclear extract

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168978

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caudal type homeobox 2 , CDX-3 , CDX2/AS , CDX3

Cellular Localization

Nucleus

Background

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CDX2 antibody used in Western Blot (WB). GTX113160

GTX113160 WB Image

Orthogonal

HCT116 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDX2 antibody (GTX113160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113160

GTX113160 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CDX2(GTX113160) antibody at 1:500 dilution.

Anti-CDX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113160

GTX113160 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal colon, using CDX2(GTX113160) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Huang HI et al. PLoS One 2018; 13 (1) : e0191617 Inhibition of EV71 by curcumin in intestinal epithelial cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

CDX2 antibody Cat. No. GTX113160

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-CDX2 antibody used in Western Blot (WB). GTX113160
Date : Anonymous submitted on 20-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : CaCo2 cells
Amount used : 10 μg
Blocking : 0.5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : Undiluted, 25°C, 12Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169