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CLIC4 antibody

Anti-CLIC4 antibody used in Western Blot (WB). GTX104888
Anti-CLIC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX104888
Anti-CLIC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104888
Anti-CLIC4 antibody used in Western Blot (WB). GTX104888
Anti-CLIC4 antibody used in Western Blot (WB). GTX104888

Cat. No. GTX104888

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CLIC4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036586

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chloride intracellular channel 4 , CLIC4L , H1 , MTCLIC , huH1 , p64H1

Cellular Localization

Cytoplasm , Cytoplasmic vesicle membrane; Single-pass membrane protein , Nucleus , Cell membrane; Single-pass membrane protein , Cytoplasm , Cytoplasmic vesicle membrane , Nucleus , Cell membrane

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CLIC4 antibody used in Western Blot (WB). GTX104888

GTX104888 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
12% SDS PAGE
GTX104888 diluted at 1:1000

Anti-CLIC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX104888

GTX104888 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using CLIC4 antibody (GTX104888) at 1:300 dilution.

Anti-CLIC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104888

GTX104888 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CLIC4(GTX104888) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-CLIC4 antibody used in Western Blot (WB). GTX104888

GTX104888 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody (GTX104888) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CLIC4 antibody used in Western Blot (WB). GTX104888

GTX104888 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody (GTX104888) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169