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COASY antibody

Anti-COASY antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107623
Anti-COASY antibody used in Western Blot (WB). GTX107623
Anti-COASY antibody used in IHC (Paraffin sections) (IHC-P). GTX107623

Cat. No. GTX107623

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all COASY products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COASY. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036610

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Coenzyme A synthase , DPCK , NBIA6 , NBP , PCH12 , PPAT , UKR1 , pOV-2

Cellular Localization

Cytoplasm

Background

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-COASY antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107623

GTX107623 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CoA synthase(GTX107623) antibody at 1:200 dilution.

Anti-COASY antibody used in Western Blot (WB). GTX107623

GTX107623 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX107623 diluted at 1:1000
Anti-COASY antibody used in IHC (Paraffin sections) (IHC-P). GTX107623

GTX107623 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using COASY(GTX107623) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169