APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5-2μg/ml |
5μg/ml |
3 - 5 μg/ml |
Note :
ICC/IF
Strong expression of the protein seen in the Golgi apparatus and vesicles of NIH3T3 cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX22338-PEP).
Product Note
This antibody is expected to recognise both human isoforms of this protein according to NP_000953.2 and NP_542158.1.
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QDDGPAVERPSTEL, from the C Terminus of the protein sequence according to NP_000953.2; NP_542158.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
prostaglandin-endoperoxide synthase 1 , COX1 , COX3 , PCOX1 , PES-1 , PGG/HS , PGHS-1 , PGHS1 , PHS1 , PTGHS
Background
This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Database
Research Area
DATA IMAGES
|
GTX22338 WB Image
WB analysis of mouse kidney (A) and skeletal muscle (B) lysate using GTX22338 COX1 antibody. Dilution : 0.5μg/ml Loading : 35μg protein in RIPA buffer
|
|
GTX22338 WB Image
WB analysis of U937 lysate using GTX22338 COX1 antibody. Dilution : 2μg/ml Loading : 35μg protein in RIPA buffer
|
|
GTX22338 IHC-P Image
IHC-P analysis of human heart using GTX22338 COX1 antibody. Antigen retrieval : citrate buffer pH 6 Dilution : 3.8μg/ml
|
|
GTX22338 ICC/IF Image
ICC/IF analysis of PFA fixed NIH-3T3 cells using GTX22338 COX1 antibody. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 5μg/ml
|
REFERENCE
There are currently no references for COX1 antibody (GTX22338). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for COX1 antibody (GTX22338). Be the first to share your experience with this product.