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CXCR2 antibody [GT547]

Anti-CXCR2 antibody [GT547] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631667
Anti-CXCR2 antibody [GT547] used in Western Blot (WB). GTX631667
Anti-CXCR2 antibody [GT547] used in Western Blot (WB). GTX631667

Cat. No. GTX631667

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT547

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CXCR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

3xFlag-human CXCR2-transfected 293T

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CXCR2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888251

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine receptor 2 , CD182 , CDw128b , CMKAR2 , IL8R2 , IL8RA , IL8RB

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CXCR2 antibody [GT547] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631667

GTX631667 ICC/IF Image

CXCR2 antibody [GT547] detects CXCR2 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR2 stained by CXCR2 antibody [GT547] (GTX631667) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-CXCR2 antibody [GT547] used in Western Blot (WB). GTX631667

GTX631667 WB Image

Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [GT547] (GTX631667) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CXCR2 antibody [GT547] used in Western Blot (WB). GTX631667

GTX631667 WB Image

CXCR2 antibody detects CXCR2 protein by western blot analysis. Non-transfected (-) and CXCR2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody (GTX631667) diluted by 1:5000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169