CaMKII (phospho Thr286) antibody [HL2908]

Cat. No. GTX640251

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2908
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all CaMKII alpha products

Datasheet

View product citations for antibody GTX640251 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr286 of human CaMKII alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium/calmodulin dependent protein kinase II alpha , CAMKA , CaMKIINalpha , MRD53 , MRT63

Cellular Localization

Cell junction, synapse,Postsynaptic density,Cell projection, dendritic spine,Cell projection, dendrite

Background

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2018]

Database

Gene ID: 815 CAMK2A
UniProt: Q9UQM7 CAMK2A

Research Area

DATA IMAGES

GTX640251 WB Image

Untreated (–) and treated (+) mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII (phospho Thr286) antibody [HL2908] (GTX640251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX640251 ICC/IF Image

CaMKII (phospho Thr286) antibody [HL2908] detects CaMKII (phospho Thr286) protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CaMKII (phospho Thr286) stained by CaMKII (phospho Thr286) antibody [HL2908] (GTX640251) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

CaMKII (phospho Thr286) antibody [HL2908]

Cat. No. GTX640251

Host

Clonality

Clone Name

Isotype

Application

Reactivity