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Caspase 1 p10 subunit antibody

Orthogonal
Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX134551
Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX134551
Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX134551
Anti-Caspase 1 p10 subunit antibody used in Western Blot (WB). GTX134551
Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX134551

Cat. No. GTX134551

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Caspase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Caspase 1 p10 subunit. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887306

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

caspase 1 , ICE , IL1BC , P45

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX134551

GTX134551 IHC-P Image

Caspase 1 p10 subunit antibody detects Caspase 1 p10 subunit protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Caspase 1 p10 subunit stained by Caspase 1 p10 subunit antibody (GTX134551) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX134551

GTX134551 IHC-P Image

Caspase 1 p10 subunit antibody detects Caspase 1 p10 subunit protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Caspase 1 p10 subunit stained by Caspase 1 p10 subunit antibody (GTX134551) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX134551

GTX134551 IHC-P Image

Caspase 1 p10 subunit antibody detects Caspase 1 p10 subunit protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Caspase 1 p10 subunit stained by Caspase 1 p10 subunit antibody (GTX134551) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 1 p10 subunit antibody used in Western Blot (WB). GTX134551

GTX134551 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 1 p10 subunit antibody (GTX134551) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX134551

GTX134551 IHC-P Image

Caspase 1 p10 subunit antibody detects Caspase 1 p10 subunit protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Caspase 1 p10 subunit stained by Caspase 1 p10 subunit antibody (GTX134551) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zhang X et al. Sci Adv 2024; 10 (2) : eadj4260 Targeting pyroptosis with nanoparticles to alleviate neuroinflammatory for preventing secondary damage following traumatic brain injury.
于 莉 et al. Research Square, 2023; Mechanism of berberine regulating ox-LDL induced endothelial pyroptosis based on TXNIP / NLRP3 / GSDMD pathway
Application : WB
Reactivity : Human
Zhang C et al. Mol Med Rep 2021; 23 (5) Cytokine secretion and pyroptosis of cholesteatoma keratinocytes mediated by AIM2 inflammasomes in response to cytoplasmic DNA.
Application : WB
Reactivity : Human
Chuang LT et al. Molecules 2021; 26 (3) Suppressive Effect of Two Cucurbitane-Type Triterpenoids from Momordica charantia on Cutibacterium acnes-Induced Inflammatory Responses in Human THP-1 Monocytic Cell and Mouse Models.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169