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Caspase 9 (cleaved Asp330) antibody

Orthogonal
Anti-Caspase 9 (cleaved Asp330) antibody used in Western Blot (WB). GTX132331
Anti-Caspase 9 (cleaved Asp330) antibody used in Western Blot (WB). GTX132331

Cat. No. GTX132331

Reference 10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Caspase 9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

Jurkat (10 μM Etoposide treatment for 24 hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Caspase 9 (cleaved Asp330). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886615

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 9 , APAF-3 , APAF3 , ICE-LAP6 , MCH6 , PPP1R56

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-Caspase 9 (cleaved Asp330) antibody used in Western Blot (WB). GTX132331

GTX132331 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 (cleaved Asp330) antibody (GTX132331) diluted at 1:500.

Anti-Caspase 9 (cleaved Asp330) antibody used in Western Blot (WB). GTX132331

GTX132331 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2019.PMID: 31341250

REFERENCE

Application Reference

Sapienza S et al. Int J Mol Sci 2022; 23 (21) Size-Based Effects of Anthropogenic Ultrafine Particles on Lysosomal TRPML1 Channel and Autophagy in Motoneuron-like Cells.
Application : WB
Xing R et al. Drug Dev Res 2021; Neuroprotective effect of Na+ /H+ exchangers isoform-1 inactivation against 6-hydroxydopamine-induced mitochondrial dysfunction and neuronal apoptosis in Parkinson's disease models.
Yan D et al. J Control Release 2020; 327 : 766-778 Co-delivery of homoharringtonine and doxorubicin boosts therapeutic efficacy of refractory acute myeloid leukemia.
Application : WB
Reactivity : Human
Wang YY et al. ACS Omega 2020; 5 (12) : 7018-7024 Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo.
Application : WB
Reactivity : Human
Cui Z et al. Clin Exp Pharmacol Physiol 2019; (Epub) MicroRNA-378-3p/5p represses proliferation and induces apoptosis of oral squamous carcinoma cells via targeting KLK4.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Sapienza S et al. Int J Mol Sci 2022; 23 (21) Size-Based Effects of Anthropogenic Ultrafine Particles on Lysosomal TRPML1 Channel and Autophagy in Motoneuron-like Cells.
Application : WB
Xing R et al. Drug Dev Res 2021; Neuroprotective effect of Na+ /H+ exchangers isoform-1 inactivation against 6-hydroxydopamine-induced mitochondrial dysfunction and neuronal apoptosis in Parkinson's disease models.
Yan D et al. J Control Release 2020; 327:766-778 Co-delivery of homoharringtonine and doxorubicin boosts therapeutic efficacy of refractory acute myeloid leukemia.
Application : WB
Reactivity : Human
Wang YY et al. ACS Omega 2020; 5 (12):7018-7024 Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo.
Application : WB
Reactivity : Human
Cui Z et al. Clin Exp Pharmacol Physiol 2019; (Epub) MicroRNA-378-3p/5p represses proliferation and induces apoptosis of oral squamous carcinoma cells via targeting KLK4.
Application : WB
Reactivity : Human
XiaotianZhang et al. Nutrition, Metabolism and Cardiovascular Diseases 2019; (Epub) MicroRNA-217-5p ameliorates endothelial cell apoptosis induced by ox-LDL by targeting CLIC4.
Tedeschi V et al. Sci Rep 2019; 9 (1):10743 The activation of Mucolipin TRP channel 1 (TRPML1) protects motor neurons from L-BMAA neurotoxicity by promoting autophagic clearance.
Application : WB
Reactivity : Rat
Dasgupta A et al. IUBMB Life 2019; (Epub) Astrakurkurone, a Sesquiterpenoid from Wild Edible Mushroom, Targets Liver Cancer Cells by Modulating Bcl-2 Family Proteins.
Application : WB
Reactivity : Human
Chen CC et al. Cancers (Basel) 2018; 10 (9) Melatonin Sensitizes Hepatocellular Carcinoma Cells to Chemotherapy Through Long Non-Coding RNA RAD51-AS1-Mediated Suppression of DNA Repair.
Application : WB
Reactivity : Human
Chen CC et al. Cell Death Dis 2018; 9 (5):543 Corylin increases the sensitivity of hepatocellular carcinoma cells to chemotherapy through long noncoding RNA RAD51-AS1-mediated inhibition of DNA repair.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169