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Cofilin 1 antibody [GT217]

KO/KD
Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582
Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582
Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582
Anti-Cofilin 1 antibody [GT217] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632582
Anti-Cofilin 1 antibody [GT217] used in IHC (Paraffin sections) (IHC-P). GTX632582
Anti-Cofilin 1 antibody [GT217] used in Immunoprecipitation (IP). GTX632582
Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582

Cat. No. GTX632582

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT217

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cofilin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 , 293T

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888319

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cofilin 1 , CFL , HEL-S-15 , cofilin

Cellular Localization

Nucleus matrix , Cytoplasm , cytoskeleton

Background

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Database

Research Area

DATA IMAGES

Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582

GTX632582 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000.

Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582

GTX632582 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582

GTX632582 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody [GT217] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632582

GTX632582 ICC/IF Image

Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cofilin 1 stained by Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Cofilin 1 antibody [GT217] used in IHC (Paraffin sections) (IHC-P). GTX632582

GTX632582 IHC-P Image

Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cofilin 1 antibody [GT217] used in Immunoprecipitation (IP). GTX632582

GTX632582 IP Image

Immunoprecipitation of Cofilin 1 protein from 293T whole cell extracts using 5 μg of Cofilin 1 antibody [GT217] (GTX632582).
Western blot analysis was performed using Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582

GTX632582 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169