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Collagen II antibody [HL1772]

Recombinant antibody Orthogonal Overexpression
Anti-Collagen II antibody [HL1772] used in Western Blot (WB). GTX637424
Anti-Collagen II antibody [HL1772] used in IHC (Paraffin sections) (IHC-P). GTX637424
Anti-Collagen II antibody [HL1772] used in Western Blot (WB). GTX637424

Cat. No. GTX637424

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1772

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Dog
Package
100 μl ($399),
25 μl ($169)
See all Collagen II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Product Note

This antibody is specific for human COL2A1 protein, and it does not cross react with human COL1A2, COL1A1, and COL3A1 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Collagen II

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type II alpha 1 chain , ANFH , AOM , COL11A3 , SEDC , STL1

Cellular Localization

Secreted, extracellular space, extracellular matrix

Background

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Collagen II antibody [HL1772] used in Western Blot (WB). GTX637424

GTX637424 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody [HL1772] (GTX637424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Collagen II antibody [HL1772] used in IHC (Paraffin sections) (IHC-P). GTX637424

GTX637424 IHC-P Image

Collagen II antibody [HL1772] detects secreted Collagen II protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog cerebellum.
Collagen II stained by Collagen II antibody [HL1772] (GTX637424) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Collagen II antibody [HL1772] used in Western Blot (WB). GTX637424

GTX637424 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody [HL1772] (GTX637424) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations