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CrkL antibody [GT466]

Anti-CrkL antibody [GT466] used in IHC (Paraffin sections) (IHC-P). GTX634534
Anti-CrkL antibody [GT466] used in Western Blot (WB). GTX634534
Anti-CrkL antibody [GT466] used in IHC (Paraffin sections) (IHC-P). GTX634534

Cat. No. GTX634534

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT466

Isotype

 IgG2b

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CrkL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CrkL. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888463

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CRK like proto-oncogene, adaptor protein

Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-CrkL antibody [GT466] used in IHC (Paraffin sections) (IHC-P). GTX634534

GTX634534 IHC-P Image

CrkL antibody [GT466] detects CrkL protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
CrkL stained by CrkL antibody [GT466] (GTX634534) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CrkL antibody [GT466] used in Western Blot (WB). GTX634534

GTX634534 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody [GT466] (GTX634534) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CrkL antibody [GT466] used in IHC (Paraffin sections) (IHC-P). GTX634534

GTX634534 IHC-P Image

CrkL antibody [GT466] detects CrkL protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CrkL stained by CrkL antibody [GT466] (GTX634534) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations