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Cyclin B1 antibody

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX22096
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX22096
Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX22096
Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX22096

Cat. No. GTX22096

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ELISA

Reactivity

 Human
Package
100 μl ($589)
See all Cyclin B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:500
IP 1:100
ELISA 1:2000-1:10000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Product Note

No reaction was observed against other related cyclins.

PROPERTIES

Form

Liquid

Buffer

Filtered-steriled serum

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Full length fusion protein corresponding to the human gene.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin B1 , CCNB

Cellular Localization

Cytoplasm,Nucleus

Background

In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at aa 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34 cdc2 rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition.

Database

Research Area

DATA IMAGES

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX22096

GTX22096 WB Image

Western blot analysis using GeneTex Anti-Cyclin B1 antibody (GTX22096) shows detection of human Cyclin B1 present in asynchronous HN30 cell lysates. HN30 cells are from head and neck cancer tumors that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~62 kDa corresponding to the expected molecular weight for the protein. The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat anti Rabbit IgG GTX27090 and chemiluminescence reagent with a 1min exposure time.

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX22096

GTX22096 WB Image

Western blot analysis using GeneTex Anti-Cyclin B1 antibody (GTX22096) shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B1 (arrowhead). Approximately 50 μg of lysate was loaded on to a 7% SDS-PAGE gel for separation. After transfer to nitrocellulose, the blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat anti Rabbit IgG (GTX27090).

Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX22096

GTX22096 IHC-P Image

GeneTex anti-Cyclin B1 antibody (GTX22096) was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX22096

GTX22096 IHC-P Image

IHC-P analysis of human brain cerebellum tissue using GTX22096 Cyclin B1 antibody.
Dilution : 1:500

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589