APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1.5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88930-PEP).
Product Note
This antibody is expected to recognise both reported isoforms (NP_004219.2; NP_059431.1).
Predict Reactivity
Mouse, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence EDGVYEPPDLTP-C, from the N Terminus of the protein sequence according to NP_004219.2; NP_059431.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytohesin 2 , ARNO , CTS18 , CTS18.1 , PSCD2 , PSCD2L , SEC7L , Sec7p-L , Sec7p-like , cytohesin-2
Cellular Localization
Cell membrane , Cytoplasm
Background
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Database
Research Area
DATA IMAGES
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GTX88930 WB Image
WB analysis of human brain (cerebellum) lysate using GTX88930 Cytohesin 2 antibody, N-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for Cytohesin 2 antibody, N-term (GTX88930). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytohesin 2 antibody, N-term (GTX88930). Be the first to share your experience with this product.
