GeneTex

  • Country / Location Selection

United States (US)

Cytokeratin 5 + 14 antibody

Cat. No. GTX40203

Host

 Mouse

Clonality

 Monoclonal

Isotype

 IgM

Application

 IHC

Reactivity

 Human
Package
100 μg ($399)
See all Cytokeratin 5 + 14 products

APPLICATION

Application Note

IHC: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Product Note

Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The 50,000 Mr band (including K14 )cut from an SDS-polyacrylamide gel and Triton X-100 extracted cytoskeleton pellet from cultured human foreskin keratinocytes.

Purification

IgM fraction

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Basal layer

Background

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis.Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.

Research Area

REFERENCE

There are currently no references for Cytokeratin 5 + 14 antibody (GTX40203). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Cytokeratin 5 + 14 antibody (GTX40203). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations