Cytokeratin 8 antibody [4D12]

Cat. No. GTX84248

Host	Mouse
Clonality	Monoclonal
Clone Name	4D12
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Dog, Monkey
Package	100 μl ($519)

See all Cytokeratin 8 products

Datasheet
Document

View product citations for antibody GTX84248 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
ICC/IF	1:100
IHC-P	1:50

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm,nucleoplasm

Background

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Database

Gene ID: 3856 KRT8
UniProt: P05787 KRT8

Research Area

DATA IMAGES

GTX84248 WB Image

WB analysis of HEK293T cells transfected with Cytokeratin 8 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84248 Cytokeratin 8 antibody [4D12].
Loading : 5 ug per lane

GTX84248 WB Image

WB analysis of wild type and Cytokeratin 8 knock out HeLa cell lysate(10μg per lane) using GTX84248 Cytokeratin 8 antibody [4D12].
Dilution : 1:1000

GTX84248 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Cytokeratin 8 plasmid using GTX84248 Cytokeratin 8 antibody [4D12].

GTX84248 ICC/IF Image

ICC/IF analysis of A549 cells using GTX84248 Cytokeratin 8 antibody [4D12].

GTX84248 WB Image

WB analysis of various cell lines using GTX84248 Cytokeratin 8 antibody [4D12].
Loading : 35 ug per lane

GTX84248 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84248 Cytokeratin 8 antibody [4D12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

Cytokeratin 8 antibody [4D12]

Cat. No. GTX84248

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84248 WB Image

GTX84248 WB Image

GTX84248 ICC/IF Image

GTX84248 ICC/IF Image

GTX84248 WB Image

GTX84248 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.