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DDAH2 antibody

Anti-DDAH2 antibody used in Western Blot (WB). GTX54028
Anti-DDAH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54028

Cat. No. GTX54028

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DDAH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH2 (NP_039268.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dimethylarginine dimethylaminohydrolase 2 , DDAH , DDAHII , G6a , HEL-S-277 , NG30

Cellular Localization

Cytoplasm,Mitochondrion

Background

This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-DDAH2 antibody used in Western Blot (WB). GTX54028

GTX54028 WB Image

WB analysis of various sample lysates using GTX54028 DDAH2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DDAH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54028

GTX54028 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54028 DDAH2 antibody.
Blue : DAPI

REFERENCE

Application Reference

Huang S et al. Sci Signal 2021; 14 (678) DDAH2 suppresses RLR-MAVS-mediated innate antiviral immunity by stimulating nitric oxide-activated, Drp1-induced mitochondrial fission.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399