APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
5μg/ml |
Note :
IHC-P
Human Adrenal Gland (Medulla) shows strong reticulate staining in the cytoplasm of medular cells.
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Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89689-PEP).
Product Note
This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3 resp) but NOT isoform 2 (NP_006085.2).
Predict Reactivity
Mouse, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence SVAIRKRSWEEHC, from the N Terminus of the protein sequence according to NP_872584.2; NP_079043.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DLC1 Rho GTPase activating protein , ARHGAP7 , HP , STARD12 , p122-RhoGAP
Cellular Localization
Cytoplasm
Background
This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.[provided by RefSeq, Apr 2010]
Database
Research Area
DATA IMAGES
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GTX89689 IHC-P Image
IHC-P analysis of human adrenal gland using GTX89689 DLC1 (Isoforms 1 and 3) antibody, N-term. Antigen retrieval : citrate buffer pH 6 Dilution : 5μg/ml
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REFERENCE
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REVIEW
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