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DNA-PKcs antibody [C3], C-term

Anti-DNA-PKcs antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109673
Anti-DNA-PKcs antibody [C3], C-term used in Western Blot (WB). GTX109673
Anti-DNA-PKcs antibody [C3], C-term used in Western Blot (WB). GTX109673
Anti-DNA-PKcs antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109673

Cat. No. GTX109673

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DNA-PKcs products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

469 kDa. ( Note )

Positive Control

293T , Molt-4 , NCI-H929

Predict Reactivity

Mouse, Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DNA-PKcs. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951440

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase, DNA-activated, catalytic subunit , DNA-PKC , DNA-PKcs , DNAPK , DNAPKc , DNPK1 , HYRC , HYRC1 , IMD26 , XRCC7 , p350

Cellular Localization

Nucleus

Background

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-DNA-PKcs antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109673

GTX109673 ICC/IF Image

DNA-PKcs antibody [C3], C-term detects DNA-PKcs protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA-PKcs protein stained by DNA-PKcs antibody [C3], C-term (GTX109673) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-DNA-PKcs antibody [C3], C-term used in Western Blot (WB). GTX109673

GTX109673 WB Image

Sample(30 ug whole cell lysate)
A:293T
B:MOLT4 (GTX27912)
5% SDS PAGE
GTX109673 diluted at 1:1000

Anti-DNA-PKcs antibody [C3], C-term used in Western Blot (WB). GTX109673

GTX109673 WB Image

DNA PKcs antibody detects DNA PKcs protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DNA PKcs antibody (GTX109673) at a dilution of 1:500.

Anti-DNA-PKcs antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109673

GTX109673 IHC-P Image

DNA-PKcs antibody [C3], C-term detects DNA-PKcs protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: DNA-PKcs antibody [C3], C-term (GTX109673) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Jang K et al. Genome Biol 2020; 21 (1) : 155 Computational inference of cancer-specific vulnerabilities in clinical samples.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169