APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Product Note
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Thr669 of mature EGFR is equivalent to Thr693 of uncleaved EGFR.
Predict Reactivity
Mouse, Rat, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
3.18 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr669 of human EGFR. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888511
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cellular Localization
Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Database
Research Area
DATA IMAGES
![Anti-EGFR (phospho Thr669) antibody [GT1361] used in Western Blot (WB). GTX635205](/templates/default/js/lightbox/images/loading.gif "Anti-EGFR (phospho Thr669) antibody [GT1361] used in Western Blot (WB). GTX635205")
|
GTX635205 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody [GT1361] (GTX635205) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for EGFR (phospho Thr669) antibody [GT1361] (GTX635205). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EGFR (phospho Thr669) antibody [GT1361] (GTX635205). Be the first to share your experience with this product.
![Anti-EGFR (phospho Thr669) antibody [GT1361] used in Western Blot (WB). GTX635205](/upload/website/prouct_img/small/GTX635205/GTX635205_43605_20190628_WB_treatment_EGF_w_23061202_116.webp "Anti-EGFR (phospho Thr669) antibody [GT1361] used in Western Blot (WB). GTX635205")
![Anti-EGFR (phospho Thr669) antibody [GT1361] used in Western Blot (WB). GTX635205](/upload/website/prouct_img/normal/GTX635205/GTX635205_43605_20190628_WB_treatment_EGF_w_23061202_116.webp "Anti-EGFR (phospho Thr669) antibody [GT1361] used in Western Blot (WB). GTX635205")