APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.1-0.3μg/ml |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89955-PEP).
Product Note
This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform.
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence PKEPSNYDFVYDCN, from the C Terminus of the protein sequence according to NP_055615.8; NP_569709.1; NP_001034548.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
engulfment and cell motility 1 , CED-12 , CED12 , ELMO-1
Cellular Localization
Cytoplasm , Cell membrane
Background
This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Database
Research Area
DATA IMAGES
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GTX89955 WB Image
WB analysis of human frontal cortex (A), mouse (B) and rat (C) brain lysate using GTX89955 ELMO1 antibody, C-term. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
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GTX89955 WB Image
WB analysis of Jurkat cell lysate using GTX89955 ELMO1 antibody, C-term. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for ELMO1 antibody, C-term (GTX89955). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ELMO1 antibody, C-term (GTX89955). Be the first to share your experience with this product.