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Ephrin B2 antibody, Internal

Orthogonal
Anti-Ephrin B2 antibody, Internal used in Western Blot (WB). GTX88049
Anti-Ephrin B2 antibody, Internal used in Western Blot (WB). GTX88049

Cat. No. GTX88049

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Ephrin B2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88049-PEP).

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CPKVDSKTVGQYE, from the internal region of the protein sequence according to NP_004084.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10725333

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ephrin B2 , EPLG5 , HTKL , Htk-L , LERK5

Cellular Localization

Cell membrane

Background

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ephrin B2 antibody, Internal used in Western Blot (WB). GTX88049

GTX88049 WB Image

WB analysis of rat lung lysate using GTX88049 Ephrin B2 antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Ephrin B2 antibody, Internal used in Western Blot (WB). GTX88049

GTX88049 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2015. PMID: 26268439

REFERENCE

Application Reference

E Oshima et al. J Neuroinflammation 2023; 20 (1) : 258 M2 macrophage-derived cathepsin S promotes peripheral nerve regeneration via fibroblast–Schwann cell-signaling relay
Caporali A et al. Nat Commun 2015; 6 : 8024 p75(NTR)-dependent activation of NF-κB regulates microRNA-503 transcription and pericyte-endothelial crosstalk in diabetes after limb ischaemia.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399