APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Human ER alpha (estrogen receptor alpha), using a synthetic peptide.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1
Cellular Localization
Cytoplasm , Cell membrane , Cell membrane; Single-pass type I membrane protein , Golgi apparatus
Background
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
Database
Research Area
DATA IMAGES
![Anti-Estrogen Receptor alpha antibody [GT9004] used in ChIP assay (ChIP assay). GTX60340](/templates/default/js/lightbox/images/loading.gif "Anti-Estrogen Receptor alpha antibody [GT9004] used in ChIP assay (ChIP assay). GTX60340")
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GTX60340 ChIP assay Image
ChIP analysis of sheared chromatin from MCF7 cells treated for 1 hour with estradiol using GTX60340 Estrogen Receptor alpha antibody [GT9004]. The IP'd DNA was analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. This figure shows the obtained peaks near the TFF1 gene on chromosome 21 (figure 1A), the GREB1 and HAAO genes on chromosome 2 (figure 1B and C), and the ZNF185 gene on the X-chromosome (figure 1D).
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REFERENCE
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REVIEW
There are currently no reviews for Estrogen Receptor alpha antibody [GT9004] (GTX60340). Be the first to share your experience with this product.
![Anti-Estrogen Receptor alpha antibody [GT9004] used in ChIP assay (ChIP assay). GTX60340](/upload/website/prouct_img/small/GTX60340/GTX60340_20201117_ChIP_64_w_23061123_131.webp "Anti-Estrogen Receptor alpha antibody [GT9004] used in ChIP assay (ChIP assay). GTX60340")
![Anti-Estrogen Receptor alpha antibody [GT9004] used in ChIP assay (ChIP assay). GTX60340](/upload/website/prouct_img/normal/GTX60340/GTX60340_20201117_ChIP_64_w_23061123_131.webp "Anti-Estrogen Receptor alpha antibody [GT9004] used in ChIP assay (ChIP assay). GTX60340")