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FLI1 antibody

Anti-FLI1 antibody used in Western Blot (WB). GTX31888
Anti-FLI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31888

Cat. No. GTX31888

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all FLI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , Jurkat Cell Lysate(GTX27899)

Product Note

At least four isoforms of FLI1 are known to exist; this antibody will detect all four.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

FLI1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human FLI1.The immunogen is located within the last 50 amino acids of FLI1 .

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fli-1 proto-oncogene, ETS transcription factor , BDPLT21 , EWSR2 , SIC-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-FLI1 antibody used in Western Blot (WB). GTX31888

GTX31888 WB Image

WB analysis of Jurkat cell lysate using GTX31888 FLI1 antibody.
Dilution : 0.5μg/ml (Lane A) and 1 μg/ml (Lane B)

Anti-FLI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31888

GTX31888 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31888 FLI1 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399