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FNBP1L antibody

Orthogonal
Anti-FNBP1L antibody used in Western Blot (WB). GTX132563
Anti-FNBP1L antibody used in Western Blot (WB). GTX132563

Cat. No. GTX132563

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FNBP1L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Lovo (200 ng/ml EGF treatment for 0.5 hr) , Jurkat (10 μM Etoposide treatment for 24hr)

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FNBP1L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886676

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

formin binding protein 1 like , C1orf39 , TOCA1

Cellular Localization

Cytoplasm , cytoskeleton , cell cortex , Cytoplasmic vesicle , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

The protein encoded by this gene binds to both CDC42 and N-WASP. This protein promotes CDC42-induced actin polymerization by activating the N-WASP-WIP complex and, therefore, is involved in a pathway that links cell surface signals to the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FNBP1L antibody used in Western Blot (WB). GTX132563

GTX132563 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FNBP1L antibody (GTX132563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FNBP1L antibody used in Western Blot (WB). GTX132563

GTX132563 WB Image

Orthogonal

Untreated (–) and treated (+) LoVo whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FNBP1L antibody (GTX132563) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169